CD20 antibody

Catalog No. ABIN6935154

The Mouse Monoclonal anti-CD20 antibody has been validated for FACS, IF and IHC (f). It is suitable to detect CD20 in samples from Human. There is 1 publication available.

Quick Overview for CD20 antibody (ABIN6935154)

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD20 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: L26-IGEL-773

Product Details anti-CD20 Antibody

Specificity: Recognizes a protein of 30-33 kDa, which is identified as CD20. Its epitope is located in the cytoplasmic domain of CD20 and was, therefore, ascribed as CD20cy in the 5th Workshop. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.

No Cross-Reactivity: Cow (Bovine), Dog (Canine), Pig (Porcine), Rat (Rattus)

Cross-Reactivity (Details): Human. Baboon. Monkey. Does not react with Cow, Dog, Pig or Rat.

Immunogen: Human tonsil B cells (L26), Recombinant human MS4A1 protein (IGEL/773)

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (5-10 µL/million cells), ,Immunofluorescence (1:50-1:100), ,Immunohistochemistry (Formalin-fixed) (1:100-1:200 for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Daudi, Raji and U266 and human lymphocytes. Lymph nodes and tonsils.

Restrictions: For Research Use only

Handling

Buffer: Tissue culture supernatant with 0.05% Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

References for anti-CD20 antibody (ABIN6935154)

Peivandi, Martens, Heitplatz, Guseva, Mueller, Martens: "Industrial Processing Induces Pericardial Patch Degeneration." in: Frontiers in surgery, Vol. 9, pp. 881433, (2022) (PubMed).

Target Details for CD20

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Alternative Name: MS4A1

Background: APY, ATOPY, B-lymphocyte antigen CD20, B-lymphocyte cell-surface antigen B1, Bp35, Fc epsilon receptor I beta chain, Fc Fragment of IgE high affinity I receptor for beta polypeptide, FCER1B, High affinity immunoglobulin epsilon receptor subunit beta, IgE Fc receptor subunit beta, IGEL, IGER, IGHER, LEU16, Leukocyte surface antigen Leu-16, Ly44, Membrane spanning 4 domains subfamily A member 2, Membrane-spanning 4-domains subfamily A member 1 (MS4A1),CD20 / MS4A1 (B-Cell Marker)
Cellular localisation: Predominantly cell surface with some cytoplasmic

Molecular Weight: 33-37kDa

Gene ID: 931, 712553

UniProt: P11836